The lab has a long-standing interest in the use of molecular libraries to answer questions relevant to enzyme structure and function, discovery of protein-protein interaction inhibitors, and examination of the human humoral immune response to viral infections.
We have used codon randomization followed by functional selections and deep sequencing to study the determinants of enzyme catalysis and substrate specificity with a focus on 尾-lactamases.
Deng, Z., Huang, W., Bakkalbasi, E., Brown, N.G., Adamski, C.J., Rice, K., Muzny, D., Gibbs, R.A., and Palzkill, T. (2012). Deep sequencing of systematic combinatorial libraries reveals 飦-lactamase sequence constraints at high resolution. J. Mol. Biol. 424: 150-167.
Sun, Z., Hu, L., Sankaran, B., Prasad, B.V.V., and Palzkill, T. (2018). Differential active site sequence requirements for NDM-1 尾-lactamase hydrolysis of carbapenem versus penicillin and cephalosporin antibiotics. Nat. Commun. 9:4524
Huang, W., Soeung, V., Boragine, D.M., and Palzkill, T. (2020). Mapping protein-protein interaction interface peptides with Jun-Fos assisted phage display and deep sequencing. ACS Synth. Biol. 9:1882-1896.
Huang, W., Soeung, V., Boragine, D.M., Hu, L., Prasad, B.V.V., Estes, M.K., Atmar, R.L., and Palzkill, T. (2020). High-resolution mapping of human norovirus antigens via genomic phage display library selections and deep sequencing. J. Virol. 95:e01495-20.
